20201006 Adult P.astreoides Metabolomic Extractions
Project: Porites Bleaching 2019
Metric and Protocol: Metabolomic extractions
Batch Number: 1
Goal
To prepare lab materials and process metabolomic extractions on adult Porites astreoides samples for the Porites Bleaching 2019 experiment using this protocol.
Laboratory preparation
- Wiped down all counter tops and fumehood with 10% bleach solution
- Labelled 3 sets of tubes for all of my samples (45 samples)
- Tube labelling system:
- #X - A = weighing vial
- #X - B = centrifuge vial
- #X - Extra = extra extraction vial
- Organized freezer samples to consolidate all 45 samples into one one freezer bag
- Printed protocols and data sheets
- Sterilize clippers and forceps (10% bleach, DI water, ethanol)
- Subset stock extraction buffer and ammonium bicarbonate solutions into 50mL falcon tubes
- Fill all “A” vials with 1 mL of extraction buffer and stored in 4°C fridge
Samples
All samples were snap frozen in liquid nitrogen and stored at -80°C.
| Vial # | Fragment ID | Timepoint | Vial + Buffer (g) | Vial + Buffer + Coral (g) |
|---|---|---|---|---|
| 1 | R8 | A1 | 1.9745 | 2.1527 |
| 5 | R37 | A1 | 1.9593 | 2.2271 |
| 15 | R36 | A1 | 1.9676 | 2.2263 |
Notes
- Started extraction on sample R40-A1, however the dounce was not clean when taken out of the autoclaved tinfoil (looked like residual soap). I put the extraction back in the freezer however I will re-start the extraction again for that sample.
- We have 10 dounces in total. This about the best way to maximize time with autoclaving the dounces between lab days or after 5 samples
Written on October 6, 2020