20201008 Adult P.astreoides Metabolomic Extractions
Project: Porites Bleaching 2019
Metric and Protocol: Metabolomic extractions
Batch Number: 2
Goal
To prepare lab materials and process metabolomic extractions on adult Porites astreoides samples for the Porites Bleaching 2019 experiment using this protocol.
Laboratory preparation
- Wiped down all counter tops and fumehood with 10% bleach solution
- Sterilize clippers and forceps (10% bleach, DI water, ethanol)
Samples
All samples were snap frozen in liquid nitrogen and stored at -80°C.
| Extraction # | Vial # | Fragment ID | Timepoint | Vial + Buffer (g) | Vial + Buffer + Coral (g) |
|---|---|---|---|---|---|
| 4 | 10 | R40 | A1 | 1.9780 | 2.2263 |
| 5 | 28 | R28 | A2 | 1.9604 | 2.2168 |
| 6 | 24 | R32 | A2 | 1.9673 | 2.1534 |
| 7 | 20 | R37 | A2 | 1.9725 | 2.2153 |
| 8 | 35 | R37 | A4 | 1.9692 | 2.1935 |
| 9 | 40 | R40 | A4 | 1.9670 | 2.2157 |
| 10 | 32 | R19 | A4 | 1.9672 | 2.1758 |
| 11 | 29 | R35 | A2 | 1.9757 | 2.2252 |
| 12 | 6 | R7 | A1 | 1.9570 | 2.2412 |
Notes
- Extracted R40-A1 from PHYS fragment
Written on October 8, 2020