20201009 Adult P.astreoides Metabolomic Extractions
Project: Porites Bleaching 2019
Metric and Protocol: Metabolomic extractions
Batch Number: 3
Goal
To prepare lab materials and process metabolomic extractions on adult Porites astreoides samples for the Porites Bleaching 2019 experiment using this protocol.
Laboratory preparation
- Autoclaved all glass ware
- Wiped down all counter tops and fumehood with 10% bleach solution
- Sterilize clippers and forceps (10% bleach, DI water, ethanol)
- Got more dry ice
Samples
All samples were snap frozen in liquid nitrogen and stored at -80°C.
| Extraction # | Vial # | Fragment ID | Timepoint | Vial + Buffer (g) | Vial + Buffer + Coral (g) |
|---|---|---|---|---|---|
| 13 | 23 | R23 | A2 | 1.9616 | 2.2285 |
| 14 | 17 | R19 | A2 | 2.0015 | 2.2592 |
| 15 | 4 | R29 | A1 | 1.9574 | 2.3254 |
| 16 | 7 | R17 | A1 | 1.9583 | 2.2258 |
| 17 | 37 | R17 | A4 | 1.9812 | 2.2642 |
| 18 | 21 | R7 | A2 | 1.9732 | 2.3900 |
| 19 | 2 | R19 | A1 | 1.9530 | 2.1588 |
| 20 | 31 | R8 | A4 | 1.9634 | 2.2368 |
| 21 | 30 | R36 | A2 | 1.9872 | 2.4290 |
| 22 | 45 | R36 | A4 | 1.9816 | 2.2090 |
Notes
- Extracted R19-A2 from PHYS fragment because the pestle broke in the douce during initial extraction
Written on October 12, 2020