Project: Porites Bleaching 2019

Goal

To test DNA and RNA extractions on adult Porites astreoides samples for the Porites Bleaching 2019 experiment using this protocol.

Samples

Today I am using 6 adult P. astreoides samples that were snap frozen in the field. All of these samples are whole fragments that are stored at -80°C.

Vial #	Timepoint	Coral ID
25	A4	R23
26	A4	R29
27	A2	R23
28	A2	R11
29	A1	R19
30	A1	R20

Quantify Results

Qubit

To test DNA quantity: Qubit

I used DNA and RNA broad range.

	DNA (ng/uL)	RNA (ng/uL)
Standard 1	175.34	398.35
Standard 2	19251.93	8705.28
25	3.86	LOW
26	22.6	12.4
27	30.8	13.6
28	9.02	13.2
29	13.5	36.2
30	7.32	25.6

Gel

To test DNA quality: Gel Electrophoresis

TapeStation

Summary:

Sample #29:

Sample #30:

Full TapeStation report can be found here