Project: Porites Bleaching 2019

Goal

To test DNA and RNA extractions on adult Porites astreoides samples for the Porites Bleaching 2019 experiment using this protocol.

Samples

Today I am using 10 adult P. astreoides samples that were snap frozen in the field. All of these samples are whole fragments that are stored at -80°C.

Vial #	Timepoint	Coral ID
37	A2	R7
38	A2	R36
39	A2	R19
40	A4	R36
41	A4	R35
42	A4	R37
43	A4	R8
44	A1	R29
45	A1	R40
46	A1	R26

Quantify Results

Qubit

To test DNA quantity: Qubit

I used DNA and RNA broad range.

	DNA (ng/uL)	RNA (ng/uL)
Standard 1	192.89	392.97
Standard 2	21333.20	8666.78
37	12.0	24.0
38	19.4	15.2
39	27.8	14.8
40	13.5	14.2
41	14.9	10.8
42	26.0	13.6
43	14.4	25.0
44	12.3	13.2
45	20.2	13.6
46	41.2	16.4

Gel

To test DNA quality: Gel Electrophoresis

TapeStation

Summary:

Full TapeStation report can be found here