Project: Porites Bleaching 2019

Goal

To test DNA and RNA extractions on adult Porites astreoides samples for the Porites Bleaching 2019 experiment using this protocol.

Samples

Today I am using 10 adult P. astreoides samples that were snap frozen in the field. All of these samples are whole fragments that are stored at -80°C.

Vial #	Timepoint	Coral ID
47	A4	R26
48	A4	R40
49	A4	R19
50	A4	R17
51	A2	R17
52	A2	R29
53	A2	R37
54	A1	R32
55	A1	R36
56	A1	R7

Quantify Results

I ran all QC with 20201120 extractions.

Qubit

To test DNA quantity: Qubit

I used DNA and RNA broad range.

	DNA (ng/uL)	RNA (ng/uL)
Standard 1	187.02	388.57
Standard 2	19924.95	9702.05
47	18.6	15.8
48	17.6	10.4
49	18.2	21.8
50	10.8	LOW
51	20.8	14.0
52	16.1	10.0
53	16.3	12.6
54	31.6	27.0
55	20.6	16.2
56	41.2	25.2

Gel

To test DNA quality: Gel Electrophoresis

TapeStation

Summary:

Full TapeStation report can be found here