Project: Porites Bleaching 2019

Goal

To test DNA and RNA extractions on adult Porites astreoides samples for the Porites Bleaching 2019 experiment using this protocol.

Samples

Today I am using 10 adult P. astreoides samples that were snap frozen in the field. All of these samples are whole fragments that are stored at -80°C.

Vial #	Timepoint	Coral ID
67	A1	R23
68	A1	R35
69	A2	R29
70	A1	R40
71	A1	R28
72	A2	R28
73	A4	R23
74	A4	R37
75	A4	R26
76	A4	R36
77	A4	R40
78	A4	R29

Quantify Results

Qubit

To test DNA quantity: Qubit

I used DNA and RNA broad range.

	DNA (ng/uL)	RNA (ng/uL)
Standard 1	185.98	397.18
Standard 2	20666.54	8670.21
67	30.8	15.0
68	24.8	15.8
69	26.6	20.6
70	9.24	LOW
71	13.7	20.6
72	8.24	11.0
73	23.8	13.4
74	33.2	19.2
75	11.8	13.0
76	14.5	14.8
77	5.06	LOW
78	14.6	18.8

Gel

To test DNA quality: Gel Electrophoresis

TapeStation

Summary:

Full TapeStation report can be found here