Project: Porites Bleaching 2019

Goal

To test DNA and RNA extractions on adult Porites astreoides samples for the Porites Bleaching 2019 experiment using this protocol.

Samples

Today I am using 8 adult P. astreoides samples that were snap frozen in the field. All of these samples are whole fragments that are stored at -80°C.

Vial #	Timepoint	Coral ID
79	A4	R29
80	A4	R40
81	A4	R26
82	A4	R36
83	A4	R37
84	A2	R28
85	A2	R29
86	A1	R40

Quantify Results

Qubit

To test DNA quantity: Qubit

I used DNA and RNA broad range.

	DNA (ng/uL)	RNA (ng/uL)
Standard 1	180.55	406.17
Standard 2	20015.72	8873.69
79	19.5	17.8
80	7.72	20.4
81	23.4	13.6
82	27.0	25.2
83	26.6	12.0
84	23.0	14.2
85	26.8	32.8
86	18.4	11.4

Gel

To test DNA quality: Gel Electrophoresis

TapeStation

Summary:

Full TapeStation report can be found here