Project: Porites Bleaching 2019

Goal

To test DNA and RNA extractions on adult Porites astreoides samples for the Porites Bleaching 2019 experiment using this protocol.

Samples

Today I am using 3 adult P. astreoides samples that were snap frozen in the field. All of these samples are whole fragments that are stored at -80°C. I am re-doing samples that previosly failed QC.

Vial #	Timepoint	Coral ID
87	A4	R26
88	A1	R40
89	A4	R17

Quantify Results

Qubit

To test DNA quantity: Qubit

I used DNA and RNA broad range.

	DNA (ng/uL)	RNA (ng/uL)
Standard 1	196.44	403.22
Standard 2	20979.74	9029.75
87	14.6	13.2
88	23.8	33.8
89	16.0	25.4

Gel

To test DNA quality: Gel Electrophoresis

TapeStation

Summary:

Full TapeStation report can be found here