20210321 Symbiont Integration Metabolomics Extractions BATCH 1
Project: Symbiont Integration
Metric and Protocol: Metabolomic extractions
Batch Number: 1
Goal
To prepare lab materials and process metabolomic extractions on multiple life stages of M. capitata samples for the Symbiont Integration experiment using the larval protocol.
Laboratory preparation
- Autoclaved all glass ware.
- Wiped down all counter tops and fumehood with 10% bleach solution.
- Obtained dry ice.
- Took aliquots of master extraction buffer and 15% ammonium bicarbonate solutions for today’s extractions.
Samples
AH Samples that were snap frozen in liquid nitrogen and stored at -80°C.
| Original Tube Label | Life Stage | Extraction Number | New Vial Label |
|---|---|---|---|
| F23 | Larvae 4 | 1 | #1-A-20210321 |
| F25 | Larvae 5 | 2 | #2-A-20210321 |
| F33 | Recruit 1 | 3 | #3-A-20210321 |
| F2 | Egg Fertilized | 4 | #4-A-20210321 |
| F12 | Larvae 1 | 5 | #5-A-20210321 |
| F9 | Larvae 1 | 6 | #6-A-20210321 |
| F6 | Embryo 1 | 7 | #7-A-20210321 |
| F1 | Egg Fertilized | 8 | #8-A-20210321 |
| F20 | Larvae 3 | 9 | #9-A-20210321 |
| F35 | Recruit 1 | 10 | #10-A-20210321 |
All samples were loaded into duplicate vials (A and B) and additional extracts were saved in the same box and stored at -80°C.
Box Label: Symbiont integration Metabolomic Extractions 20210321 - KW/AH/Putnam Box 1
Freezer: New grey/blue -80°C
Written on March 22, 2021