20210325 Symbiont Integration Metabolomics Extractions BATCH 2
Project: Symbiont Integration
Metric and Protocol: Metabolomic extractions
Batch Number: 2
Goal
To prepare lab materials and process metabolomic extractions on multiple life stages of M. capitata samples for the Symbiont Integration experiment using the larval protocol.
Laboratory preparation
- Autoclaved all glass ware.
- Wiped down all counter tops and fumehood with 10% bleach solution.
- Obtained dry ice.
- Took aliquots of master extraction buffer and 15% ammonium bicarbonate solutions for today’s extractions.
Samples
AH Samples that were snap frozen in liquid nitrogen and stored at -80°C.
| Original Tube Label | Life Stage | Extraction Number | Extraction Buffer Volume (uL) | New Vial Label |
|---|---|---|---|---|
| F16 | Larvae 2 | 11 | 1500 | #11-A-20210325 |
| F4 | Egg Fert | 12 | 1500 | #12-A-20210325 |
| F28 | Larvae 5 | 13 | 1500 | #13-A-20210325 |
| F32 | Larvae 6 | 14 | 1000 | #14-A-20210325 |
| F27 | Larvae 5 | 15 | 1000 | #15-A-20210325 |
| F31 | Larvae 6 | 16 | 1000 | #16-A-20210325 |
| F19 | Larvae 3 | 17 | 1500 | #17-A-20210325 |
| F3 | Egg Fert | 18 | 1500 | #18-A-20210325 |
| F10 | Larvae 1 | 19 | 1500 | #19-A-20210325 |
I noticed some samples had low pigment coloration (Larvae 5/6/recruits) possibly due to low sample input. Therefore, I reduced the amount of extraction buffer to potentially increase the signal intensity.
All samples were loaded into duplicate vials (A and B) and additional extracts were saved in the same box and stored at -80°C.
Box Label: Symbiont integration Metabolomic Extractions 20210321 - KW/AH/Putnam Box 1
Freezer: New grey/blue -80°C