Project: Mcap2020

Goal

Dilute extracted RNA to 13 ug/uL in 50 uL and consolidate into one plate for Tag-seq. Molecular QC information can be found here.

Samples

Sample	RNA ng/ul average	Sample Input for 13ng/ul (uL)	Dilutent Vol (uL)	Total Vol (uL)	Well	Sample Name
D1	180	3.61	46.39	50	A1	AH1
D10	126	5.16	44.84	50	B1	AH2
D11	148	4.39	45.61	50	C1	AH3
D12	84.2	7.72	42.28	50	D1	AH4
D13	94.7	6.86	43.14	50	E1	AH5
D14	88.8	7.32	42.68	50	F1	AH6
D15	66.4	9.79	40.21	50	G1	AH7
D16	96	6.77	43.23	50	H1	AH8
D17	49.8	13.05	36.95	50	A2	AH9
D18	55.1	11.80	38.20	50	B2	AH10
D19	49.6	13.10	36.90	50	C2	AH11
D2	250	2.60	47.40	50	D2	AH12
D20	68.1	9.54	40.46	50	E2	AH13
D21	53.6	12.13	37.87	50	F2	AH14
D22	94.6	6.87	43.13	50	G2	AH15
D23	29.1	22.34	27.66	50	H2	AH16
D24	32.1	20.25	29.75	50	A3	AH17
D29	28.3	22.97	27.03	50	B3	AH18
D3	561	1.16	48.84	50	C3	AH19
D30	22.8	28.51	21.49	50	D3	AH20
D31	19.9	32.66	17.34	50	E3	AH21
D32	14.35	45.30	4.70	50	F3	AH22
D36	13	50.00	0.00	50	G3	AH23
D37	36.1	18.01	31.99	50	H3	AH24
D38	18.1	35.91	14.09	50	A4	AH25
D4	277	2.35	47.65	50	B4	AH26
D40	17.6	36.93	13.07	50	C4	AH27
D5	158.5	4.10	45.90	50	D4	AH28
D6	140	4.64	45.36	50	E4	AH29
D7	177	3.67	46.33	50	F4	AH30
D8	118	5.51	44.49	50	G4	AH31
D9	105	6.19	43.81	50	H4	AH32
Plug_10	53.3	12.20	37.80	50	A5	AH33
Plug_11	31.9	20.38	29.62	50	B5	AH34
Plug_2	40.9	15.89	34.11	50	C5	AH35
Plug_4	80.1	8.11	41.89	50	D5	AH36
Plug_5	77.5	8.39	41.61	50	E5	AH37
Plug_6	27.2	23.90	26.10	50	F5	AH38
Plug_9	75	8.67	41.33	50	G5	AH39

Plate Map

Protocol

1) Remove samples from the -80°C freezer and place on a tube rack in an ice bucket to thaw.

2) Fill each well of the 96-well PCR plate with the corresponding diluent volume (I used Ultra Pure Water).

3) Place plate onto a block frozen at -80°C.

4) Once samples are thawed, spun down, and pipette mixed the sample before adding the appropriate volume into the corresponding well.

5) Seal plate with aluminum foil tape and seal each well. Will re-label the plate once we have the JA number and shipping information.

6) Place remaining samples and plate back into the -80°C freezer.