DNA extractions for Porites Thermal Transplant ADULTS BATCH #4

Goal

To process DNA for Porites astreoides adult homogenates for the Thermal Transplant 2018 experiment using this protocol.

Samples

All samples were snap frozen, then airbrushed with filtered seawater. 0.5 mL of the homogenate was aliquoted then re-frozen at -80 °C.

Vial	Sample Type	Year	Coral ID
17-251	Adult Homogenate	2017	R-18
17-296	Adult Homogenate	2017	R-7
17-539	Adult Homogenate	2017	P-9
17-619	Adult Homogenate	2017	P-15
17-692	Adult Homogenate	2017	R-14
18-9	Adult Homogenate	2018	P-4-B
18-67	Adult Homogenate	2018	P-14-B
18-91	Adult Homogenate	2018	R-14-B
18-310	Adult Homogenate	2018	P-10-B
18-322	Adult Homogenate	2018	P-9-A

Quantify Results

Qubit

To test DNA quantity: Qubit

DNA: Broad Range

	DNA (ng/uL)
Standard 1	174.44
Standard 2	18472.23
17-251	28.4
17-296	32.8
17-539	25.8
17-619	20.0
17-692	25.6
18-9	13.8
18-67	13.6
18-91	11.1
18-310	16.1
18-322	7.18

Gel Electrophoresis

To test DNA quality: Gel Electrophoresis

• Today I did a small gel of the above protocol and ran it at 100V.