DNA extractions for Porites Thermal Transplant ADULTS BATCH #5

Goal

To process DNA for Porites astreoides adult homogenates for the Thermal Transplant 2018 experiment using this protocol.

Samples

All samples were snap frozen, then airbrushed with filtered seawater. 0.5 mL of the homogenate was aliquoted then re-frozen at -80 °C.

Vial	Sample Type	Year	Coral ID
18-20	Adult Homogenate	2018	R-7-B
18-32	Adult Homogenate	2018	P-12-B
18-154	Adult Homogenate	2018	R-18-A
18-190	Adult Homogenate	2018	P-14-A
18-226	Adult Homogenate	2018	R-11-A
18-238	Adult Homogenate	2018	R-8-B
17-9	Adult Homogenate	2017	P-3
17-27	Adult Homogenate	2017	P-12
17-529	Adult Homogenate	2017	P-6
17-540	Adult Homogenate	2017	P-9

Quantify Results

Qubit

To test DNA quantity: Qubit

DNA: Broad Range

Vial	DNA (ng/uL)
Standard 1	173.49
Standard 2	17814.16
18-20	30.2
18-32	13.0
18-154	14.6
18-190	16.2
18-226	25.6
18-238	19.1
17-9	5.84
17-27	26.0
17-529	45.6
17-540	36.4

Gel Electrophoresis

To test DNA quality: Gel Electrophoresis

• Today I did a small gel of the above protocol and ran it at 100V.