DNA extractions for Porites Thermal Transplant ADULTS BATCH #6

Goal

To process DNA for Porites astreoides adult homogenates for the Thermal Transplant 2018 experiment using this protocol.

Samples

All samples were snap frozen, then airbrushed with filtered seawater. 0.5 mL of the homogenate was aliquoted then re-frozen at -80 °C.

Vial	Sample Type	Year	Coral ID
18-44	Adult Homogenate	2018	R-19-B
18-79	Adult Homogenate	2018	R-14-A
18-106	Adult Homogenate	2018	P-19-A
18-167	Adult Homogenate	2018	R-19-A
18-214	Adult Homogenate	2018	R-18-B
18-250	Adult Homogenate	2018	R-11-B
18-262	Adult Homogenate	2018	P-15-B
18-274	Adult Homogenate	2018	P-3-B
18-286	Adult Homogenate	2018	P-16-B
18-334	Adult Homogenate	2018	P-19-B

Quantify Results

Qubit

To test DNA quantity: Qubit

DNA: Broad Range

	DNA (ng/uL)
Standard 1	191.61
Standard 2	19866.04
18-44	27.2
18-79	19.1
18-106	28.0
18-167	15.1
18-214	6.30
18-250	13.7
18-262	15.5
18-274	5.70
18-286	18.6
18-334	11.9

Gel Electrophoresis

To test DNA quality: Gel Electrophoresis

• Today I did a small gel of the above protocol and ran it at 100V.