DNA extractions for Porites Thermal Transplant ADULTS BATCH #7

Goal

To process DNA for Porites astreoides adult homogenates for the Thermal Transplant 2018 experiment using this protocol.

Samples

All samples were snap frozen, then airbrushed with filtered seawater. 0.5 mL of the homogenate was aliquoted then re-frozen at -80 °C. New samples indicate first extraction for that colony, redo indicates secondary extraction for that colony because the initial extraction had poor yields.

Vial	Sample Type	Year	Coral ID	New/Re-do
18-55	Adult Homogenate	2018	R-7-A	New
18-118	Adult Homogenate	2018	P-6-A	New
18-130	Adult Homogenate	2018	R-5-A	New
18-178	Adult Homogenate	2018	R-5-B	New
18-202	Adult Homogenate	2018	P-4-A	New
18-298	Adult Homogenate	2018	R-4-A	New
18-346	Adult Homogenate	2018	P-6-B	New
17-278	Adult Homogenate	2017	R-8	Re-do
17-305	Adult Homogenate	2017	R-15	Re-do
17-520	Adult Homogenate	2017	P-19	New
17-629	Adult Homogenate	2017	P-10	Re-do
17-656	Adult Homogenate	2017	R-9	Re-do
17-683	Adult Homogenate	2017	R-19	New

Quantify Results

Qubit

To test DNA quantity: Qubit

DNA: Broad Range

	DNA (ng/uL)
Standard 1	173.92
Standard 2	19653.52
18-55	24.4
18-118	25.6
18-130	19.2
18-178	8.42
18-202	21.6
18-298	11.3
18-346	15.3
17-278	9.02
17-305	12.8
17-520	20.0
17-629	18.5
17-656	39.2
17-683	28.6

Gel Electrophoresis

To test DNA quality: Gel Electrophoresis

• Today I did a small gel of the above protocol and ran it at 100V.