DNA Extractions for adult homogenates of Porites Thermal Transplant Batch 8
DNA extractions for Porites Thermal Transplant ADULTS BATCH #8
Goal
To process DNA for Porites astreoides adult homogenates for the Thermal Transplant 2018 experiment using this protocol.I am re-extracting 3 samples that had low quality.
Samples
All samples were snap frozen, then airbrushed with filtered seawater. 0.5 mL of the homogenate was aliquoted then re-frozen at -80 °C. New samples indicate first extraction for that colony, redo indicates secondary extraction for that colony because the initial extraction had poor yields.
| Vial | Sample Type | Year | Coral ID | New/Re-do |
|---|---|---|---|---|
| 18-227 | Adult Homogenate | 2018 | R-11-A | Re-do |
| 18-239 | Adult Homogenate | 2018 | R-8-B | Re-do |
| 18-311 | Adult Homogenate | 2018 | P-10-B | Re-do |
Quantify Results
Qubit
To test DNA quantity: Qubit
DNA: Broad Range
| DNA (ng/uL) | |
|---|---|
| Standard 1 | 191.39 |
| Standard 2 | 22110.47 |
| 18-227 | 29.2 |
| 18-239 | 20.0 |
| 18-311 | 18.7 |
Gel Electrophoresis
To test DNA quality: Gel Electrophoresis
- Today I did a small gel of the above protocol and ran it at 100V.
Written on February 8, 2021