Goal

Processing DNA/RNA extractions for Porites astreoides LARVAE: Batch #2

Samples

Today I am using 6 larval P. astreoides samples that were snap frozen in the field.

Vial	Sample Type	Coral ID
L-936	Larvae	P-19-B
L-1032	Larvae	R-14-B
L-1038	Larvae	R-8-A
L-1050	Larvae	P-14-B
L-1059	Larvae	R-15-A
L-1075	Larvae	P-6-B

Protocol description

I followed this protocol.

Quantify Results

Qubit

To test DNA quantity: Qubit

I used DNA and RNA broad range.

	DNA (ng/uL)	RNA (ng/uL)
Standard 1	174.44	377.56
Standard 2	18472.23	8989.06
L-936	38.6	38.4
L-1032	44.2	81.4
L-1038	71.8	59.4
L-1050	49.2	45.4
L-1059	88.6	85.2
L-1075	35.0	45.8

Gel

To test DNA quality: Gel Electrophoresis

TapeStation

Sample #936:

Sample #1032:

Sample #1038:

Sample #1050:

Sample #1059:

Sample #1075: