Goal

Processing DNA/RNA extractions for Porites astreoides LARVAE: Batch #3

Samples

Today I am using 6 larval P. astreoides samples that were snap frozen in the field.

Vial	Sample Type	Coral ID
L-658	Larvae	P-15-A
L-862	Larvae	P-14-A
L-1035	Larvae	P-10-A
L-1044	Larvae	P-15-B
L-1263	Larvae	R-7-A
L-1273	Larvae	R-7-B

Protocol description

I followed this protocol.

Quantify Results

Qubit

To test DNA quantity: Qubit

I used DNA and RNA broad range.

Vial	DNA (ng/uL)	RNA (ng/uL)
Standard 1	173.49	384.32
Standard 2	17814.16	9104.71
L-658	25.8	45.6
L-862	34.0	48.8
L-1035	13.9	44.4
L-1044	30.8	38.6
L-1263	53.4	79.4
L-1273	24.4	66.8

Gel

To test DNA quality: Gel Electrophoresis

TapeStation

Sample #658:

Sample #862:

Sample #1035:

Sample #1044:

Sample #1263:

Sample #1273: