Goal

Processing DNA/RNA extractions for Porites astreoides LARVAE: Batch #4

Samples

Today I am using 12 larval P. astreoides samples that were snap frozen in the field.

Vial	Sample Type	Coral ID
L-525	Larvae	P-3-A
L-562	Larvae	R-5-A
L-661	Larvae	R-9-A
L-728	Larvae	P-6-A
L-894	Larvae	P-10-B
L-909	Larvae	P-12-B
L-924	Larvae	P-9-A
L-927	Larvae	P-16-A
L-1029	Larvae	P-4-A
L-1041	Larvae	R-9-B
L-1084	Larvae	R-19-B
L-1093	Larvae	R-19-A

Protocol description

I followed this protocol.

Quantify Results

Qubit

To test DNA quantity: Qubit

I used DNA and RNA broad range.

	DNA (ng/uL)	RNA (ng/uL)
Standard 1	191.61	382.99
Standard 2	19866.04	8991.40
L-525	7.02	26.0
L-562	31.4	56.8
L-661	26.2	59.6
L-728	39.2	47.6
L-894	20.2	40.2
L-909	15.7	24.4
L-924	34.4	58.8
L-927	17.5	30.4
L-1029	42.2	41.6
L-1041	32.8	43.4
L-1084	46.6	51.8
L-1093	65.8	84.2

Gel

To test DNA quality: Gel Electrophoresis

TapeStation

Sample #525:

Sample #562:

Sample #661:

Sample #728:

Sample #894:

Sample #909:

Sample #924:

Sample #927:

Sample #1029:

Sample #1041:

Sample #1084:

Sample #1093: