Thermal Stress

Thermal Stress test for Galaxea fasicularis TEM imaging:

Tank prep

• One mesocosm raceway with two wave pumps and an independent sump with three heaters connected to the Apex system.
• One 5 gallon tank with a mini water pump and two lines for water input

20230316

• Colony D was cut into three pieces (~5cm x 5cm) and glued to square pcv plates.
• The three fragments were immediately fed with ReefRoids and left to acclimate/recover in the raceway

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• The tank was cleaned and the water was drained/refilled
• One of the three fragments was placed in the glass 5 gallon tank once temperatures returned back to 28C
• 1 photograph was taken with the colored ruler for bleaching score
• Light was recorded at 96 PAR
• Apex settings for the 3 heaters were set to as followed:
  • On temperature: 28.5
  • Off tempeature: 29.0
  • Time set: 14:00
• We are aiming for a 1 degree increase per day until we reach 35C

20230322

• Apex settings for the 3 heaters were set to as followed:
  • On temperature: 29.5
  • Off tempeature: 30.0
  • Time set: 14:27

20230323

• Apex settings for the 3 heaters were set to as followed:
  • On temperature: 30.5
  • Off tempeature: 31.0
  • Time set: 14:00

20230324

• Apex settings for the 3 heaters were set to as followed:
  • On temperature: 31.5
  • Off tempeature: 32.0
  • Time set: 15:00
• Cleaned tank

20230325

• Apex settings for the 3 heaters were set to as followed:
  • On temperature: 32.5
  • Off tempeature: 33.0
  • Time set: 14:08

20230326

• Apex settings for the 3 heaters were set to as followed:
  • On temperature: 33.5
  • Off tempeature: 34.0
  • Time set: 13:15

20230327

• Apex settings for the 3 heaters were set to as followed:
  • On temperature: 33.5
  • Off tempeature: 34.0
  • Incoming water was reduced as the tank temperatures were not reaching 34C. Seems to have worked.

20230328

• Apex settings for the 3 heaters were set to as followed:
  • On temperature: 33.5
  • Off tempeature: 34.0
  • Cleaned tank

20230329

• Apex settings for the 3 heaters were set to as followed:
  • On temperature: 33.5
  • Off tempeature: 34.0

20230330

• Apex settings for the 3 heaters were set to as followed:
  • On temperature: 33.5
  • Off tempeature: 34.0

20230331

• Apex settings for the 3 heaters were set to as followed:
  • On temperature: 33.5
  • Off tempeature: 34.0