DNA RNA Extractions on P. astreoides larvae - BEAD BEATING Batch 5

Lab work·Project Porites Thermal Transplant·Protocol DNA/RNA co-extraction from coral tissue (Zymo Quick-DNA/RNA Miniprep Plus)

Goal

Processing DNA/RNA extractions for Porites astreoides LARVAE: Batch #5

• Re-extracting sample replicates that had poor DNA or RNA quality from previous extractions

Samples

Today I am using 9 larval P. astreoides samples that were snap frozen in the field.

Vial	Sample Type	Coral ID
L-502	Larvae	P-3-A
L-571	Larvae	R-11-A
L-704	Larvae	P-15-A
L-843	Larvae	P-19-B
L-859	Larvae	P-16-A
L-933	Larvae	P-10-A
L-1081	Larvae	R-14-B
L-1260	Larvae	P-6-B
L-1266	Larvae	P-15-B

Protocol description

I followed this protocol.

Quantify Results

Qubit

To test DNA quantity: Qubit

I used DNA and RNA broad range.

	DNA (ng/uL)	RNA (ng/uL)
Standard 1	186.45	375.15
Standard 2	20244.20	9098.41
L-502	7.16	22.8
L-571	30.2	41.8
L-704	19.9	35.2
L-843	30.0	33.4
L-859	17.4	24.4
L-933	36.2	44.4
L-1081	46.2	55.2
L-1260	9.68	20.2
L-1266	16.3	26.8

Gel

To test DNA quality: Gel Electrophoresis

Gel

TapeStation

Sample #502:

Sample #571:

Sample #704:

Sample #843:

Sample #859:

Sample #933:

Sample #1081:

Sample #1260:

Sample #1266: